Transfection by Electroporation

Transfection of primary CNS and PNS neurons by electroporation

Transfection of primary human skin fibroblasts by electroporation.

Primary human skin fibroblasts are an accessible source of phenotypically and karyotypically normal human cells, but are difficult to transfect with exogenous DNA. Here we demonstrate that both transient expression and stable transformation can be carried out by the method of electroporation. Highly efficient transient chloramphenicol acetyltransferase expression was shown after transfection wi...

Size Specific Transfection to Mammalian Cells by Micropillar Array Electroporation

Electroporation serves as a promising non-viral gene delivery approach, while its current configuration carries several drawbacks associated with high-voltage electrical pulses and heterogeneous treatment on individual cells. Here we developed a new micropillar array electroporation (MAE) platform to advance the electroporation-based delivery of DNA and RNA probes into mammalian cells. By intro...

Improved transfection efficiency of 293 cells by radio frequency electroporation.

Electroporation, a widely used technique allowing the transfer of biological molecules into a variety of cell types (13), has been used with success for introducing plasmids and short oligonucleotides into mammalian, yeast and a variety of other cell types (1,2,9,10). A modification of the square-wave pulse technique that uses radio frequency (RF) electrical bursts is now being used effectively...

D-Glucosamine Promotes Transfection Efficiency during Electroporation

D-Glucosamine is a useful medicament in various fields of medicine and dentistry. With respect to stability of the cell membrane, it has been reported that bradykinin-induced nociceptive responses are significantly suppressed by the direct application of D-glucosamine. Electroporation is usually used to effectively introduce foreign genes into tissue culture cells. Buffers for electroporation w...